A Comprehensive Review of the Forensic Microbiome and its Application in Postmortem Interval Estimation
The estimation of the postmortem interval (PMI) remains one of the most significant challenges in forensic science. Traditional methods, while foundational, are often limited by environmental factors and a narrow effective time frame. The emergence of high-throughput sequencing technologies has opened a new frontier in forensic investigation through the study of the thanatomicrobiome the microbial […]
Purification and Kinetics of lignin peroxidase from Emericella nidulans
Lignin peroxidase (EC 1.11.1.14) is a potent, heme-containing enzyme that catalyzes oxidative reactions in the presence of hydrogen peroxide. Its wide-ranging capabilities stem from its effectiveness in degrading xenobiotics and various compounds with both phenolic and non-phenolic structures. The enzyme was isolated and purified from Emericella nidulans using ammonium sulfate precipitation, DEAE-cellulose and Sephadex G200. The specific […]
Natural Nano-Preservative Strategy for Reducing Nitrosamines and Enhancing the Physical Quality of Grilled Beef Kofta
This current study evaluates the efficiency of zinc oxide nanoparticles (ZnO NP) and plant powders for degrading nitrosamine compounds towards improved beef kofta quality. With consumers still demanding healthier and more natural food, the current study endeavors to explore alternative solutions other than artificial additives that are employed in the majority of meat processing. The […]
Terpene scaffolds as allosteric inhibitors of PTP1B: An integrated docking, MD, and MM/PBSA study
Protein tyrosine phosphatase 1B (PTP1B) is a validated antidiabetic and anti-obesity target whose activity can be attenuated by small molecules engaging an allosteric cleft between helices α3/α7 beneath the Phe196/Phe280 “aromatic clamp.” Motivated by the tractability of terpene scaffolds, we evaluated artemisinic acid, dehydroabietic acid, and santonin as PTP1B allosteric inhibitors using an integrated in-silico […]
Potential application of prickly pear vinegar as a surface disinfectant: in vitro and molecular docking studies
The surface disinfectant property of a homemade prickly pear vinegar (PPV) is investigated throughout this study, using in vitro testes assisted by a molecular docking study, against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Salmonella enterica) strains. Results show that PPV exhibited growth-inhibitory effect for all tested bacterial strains at MIC values ranging between 1.56, and […]